32P-labeling test for DNA damage.

Internal 32P-labeling of L-deoxyoligonucleotides.

A general two step procedure for the internal labeling of L-deoxyoligonucleotides, Spiegelmers, has been developed. Through radioactive labeling oligonucleotides can easily be detected and monitored in biological samples. T4 polynucleotide kinase is shown to efficiently phosphorylate strands of L-nucleic acids which allows the labeling with phosphorous isotopes such as (32)P. In order to protec...

Observations on the 32P uptake test.

The results of the 32P uptake test in the diagnosis of intraocular malignancies is presented. The tests were carried out with the use of newly developed eye probe. A quantitative comparison of different control sites is described, and a statistical analysis has been conducted on the counts obtained during the uptake procedure.

DNA damage in the stomach after vagotomy measured by 32P-postlabelling.

This study analysed gastric mucosal DNA by 32P-postlabelling in a series of patients who have had previous vagotomy for benign peptic ulcer disease. DNA adduct levels were found to be significantly higher in patients who had had previous truncal vagotomy than in those who had had previous highly selective vagotomy (p < 0.001). Intragastric bile concentrations were also considerably higher in pa...

Radiation track and DNA damage

A new chemical procedure for 32P-labeling of ribonucleic acids at their 5'-ends after isolation.

A new technique, which utilizes the chemical reaction between [32P]diimidazolidate of orthophosphate and the cetyltrimethylammonium salt of high-molecular-weight RNA in nonaqueous dimethyl formamide, has been developed for the 32P-labeling of RNAs after isolation. The radioactive label of high specific activity is introduced onto a phosphorylated 5'-end of the RNA and renders it suitable for 5'...